Single-particle imaging without symmetry constraints at an X-ray free-electron laser
Category
Published on
Type
journal-article
Author
Max Rose and Sergey Bobkov and Kartik Ayyer and Ruslan P. Kurta and Dmitry Dzhigaev and Young Yong Kim and Andrew J. Morgan and Chun Hong Yoon and Daniel Westphal and Johan Bielecki and Jonas A. Sellberg and Garth Williams and Filipe R.N.C. Maia and Olexander M. Yefanov and Vyacheslav Ilyin and Adrian P. Mancuso and Henry N. Chapman and Brenda G. Hogue and Andrew Aquila and Anton Barty and Ivan A. Vartanyants
Citation
Rose, M. et al., 2018. Single-particle imaging without symmetry constraints at an X-ray free-electron laser. IUCrJ, 5(6). Available at: http://dx.doi.org/10.1107/s205225251801120x.
Abstract
The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
