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Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source

By Mehmet Gul, Esra Ayan, Ebru Destan, J. Austin Johnson, Alaleh Shafiei, Abdullah Kepceoğlu, Merve Yilmaz, Fatma Betül Ertem, İlkin Yapici, Bilge Tosun, Nilüfer Baldir, Nurettin Tokay, Zeliş Nergiz, Gözde Karakadioğlu, Seyide Seda Paydos, Cahine Kulakman, Cengiz Kaan Ferah, Ömür Güven, Necati Atalay, Enver Kamil Akcan, Haluk Cetinok, Nazlı Eylül Arslan, Kardelen Şabanoğlu, Bengisu Aşci, Serra Tavli, Helin Gümüsboğa, Sevde Altuntaş, Masami Otsuka, Mikako Fujita, Şaban Teki̇n, Halilibrahim Çi̇ftçi̇, Serdar Durdaği, Ezgi Karaca, Burcu Kaplan Türköz, Burak Veli Kabasakal, Ahmet Kati, Hasan DeMi̇rci̇

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Category

BioXFEL Publications

Published on

Type

journal-article

Author

Mehmet Gul and Esra Ayan and Ebru Destan and J. Austin Johnson and Alaleh Shafiei and Abdullah Kepceoğlu and Merve Yilmaz and Fatma Betül Ertem and İlkin Yapici and Bilge Tosun and Nilüfer Baldir and Nurettin Tokay and Zeliş Nergiz and Gözde Karakadioğlu and Seyide Seda Paydos and Cahine Kulakman and Cengiz Kaan Ferah and Ömür Güven and Necati Atalay and Enver Kamil Akcan and Haluk Cetinok and Nazlı Eylül Arslan and Kardelen Şabanoğlu and Bengisu Aşci and Serra Tavli and Helin Gümüsboğa and Sevde Altuntaş and Masami Otsuka and Mikako Fujita and Şaban Teki̇n and Halilibrahim Çi̇ftçi̇ and Serdar Durdaği and Ezgi Karaca and Burcu Kaplan Türköz and Burak Veli Kabasakal and Ahmet Kati and Hasan DeMi̇rci̇

Citation

Gul, M., Ayan, E., Destan, E., Johnson, J. A., Shafiei, A., Kepceoğlu, A., Yilmaz, M., Ertem, F. B., Yapici, İ., Tosun, B., Baldir, N., Tokay, N., Nergiz, Z., Karakadioğlu, G., Paydos, S. S., Kulakman, C., Ferah, C. K., Güven, Ö., Atalay, N., … DeMi̇rci̇, H. (2023). Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-023-33989-0

Abstract

AbstractHigh-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 Å and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.

DOI

http://dx.doi.org/10.1038/s41598-023-33989-0

Funding

NSF-STC Biology with X-ray Lasers (NSF-1231306)