Practical considerations for the analysis of time-resolved x-ray data
Category
Published on
Type
journal-article
Author
Marius Schmidt
Citation
Schmidt, M. (2023). Practical considerations for the analysis of time-resolved x-ray data. Structural Dynamics, 10(4). https://doi.org/10.1063/4.0000196
Abstract
The field of time-resolved macromolecular crystallography has been expanding rapidly after free electron lasers for hard x rays (XFELs) became available. Techniques to collect and process data from XFELs spread to synchrotron light sources. Although time-scales and data collection modalities can differ substantially between these types of light sources, the analysis of the resulting x-ray data proceeds essentially along the same pathway. At the base of a successful time-resolved experiment is a difference electron density (DED) map that contains chemically meaningful signal. If such a difference map cannot be obtained, the experiment has failed. Here, a practical approach is presented to calculate DED maps and use them to determine structural models.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
