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2015 HWI Crystallization Workshop

By Tom Grant1, Bill Bauer1, Edward Snell2

1. Hauptman-Woodward Institute 2. University at Buffalo

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Workshops

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Abstract

Crystallization is the major bottleneck to 3D structure determination using X-ray crystallography. In this workshop we will discuss many tenets of successful crystallization for both conventional and serial crystallography. Many topics will be covered including fundamentals of crystal growth, strategies to sample crystallization space, identifying crystal hits, electron microscopy applications of crystal analysis, practical considerations for data analysis, difficult crystallization problems, virus crystallography, and an overview of serial crystallography.

You can view the complete playlist of videos here, or scroll down below for the full workshop series.

In This Workshop

  1. Fundamentals of Crystal Growth

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    14 Oct 2015 | Teaching Materials | Contributor(s): Edward Snell, Eaton E Lattman

    Dr. Ed Lattman discusses the foundations of crystallography and theoretical and practical considerations including X-ray radiation and production and crystallization nucleation, growth and optimization.

  2. Strategies to sample crystallization space

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    14 Oct 2015 | Teaching Materials | Contributor(s): Joseph Luft

    Joe Luft discusses crystallization screening strategies to maximize the likelihood of success.

  3. What is the outcome of your crystallization experiment

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    14 Oct 2015 | Teaching Materials | Contributor(s): Joseph Luft

    Joe Luft discusses the detection of hits in crystallization screening experiments for both macro and nanocrystals.

  4. Applications of TEM for Evaluation and Optimization of Crystal Growth

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    14 Oct 2015 | Teaching Materials | Contributor(s): Guillermo Calero

    Dr. Guillero Calero discusses using transmission electron microscopy to characterize, evaluate and subsequently optimize the growth of crystals.

  5. Practical Considerations for Crystallographic Data Analysis

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    14 Oct 2015 | Teaching Materials | Contributor(s): Tom Grant

    Dr. Thomas Grant explains how crystal quality affects diffraction data and how you can detect and ameliorate problems in crystallographic data.

  6. Optimizing crystallization hits - small to large and large to small

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    14 Oct 2015 | Teaching Materials | Contributor(s): Joseph Luft

    Joe Luft describes methods for optimizing initial results from crystallization screening trials for both macro and nanocrystals.

  7. When Your Crystals Bite

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    14 Oct 2015 | Teaching Materials | Contributor(s): George Phillips

    Dr. George Phillips describes properties of crystals such as twinning and other defects and how they can adversely affect crystallographic analysis.

  8. Virus Crystallography

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    14 Oct 2015 | Teaching Materials | Contributor(s): Robert M Lawrence

    Dr. Rob Lawrence describes the unique problems associated with crystallization of virus particles and different methods to overcome them.

  9. Overview of Serial Femtosecond Crystallography

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    14 Oct 2015 | Teaching Materials | Contributor(s): Petra Fromme

    Dr. Petra Fromme discusses an introductory overview to the field of serial crystallography and recent results showing the potential power of the technique.

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