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Cell-free production of a functional oligomeric form of aChlamydiamajor outer-membrane protein (MOMP) for vaccine development
24 Jul 2017 | BioXFEL Publications | Contributor(s): Wei He, Martina Felderman, Angela C. Evans, Jia Geng, David Homan, Feliza Bourguet, Nicholas O. Fischer, Yuanpei Li, Kit S. Lam, Aleksandr Noy, Li Xing, R. Holland Cheng, Amy Rasley, Craig D. Blanchette, Kurt Kamrud, Nathaniel Wang, Heather Gouvis, Todd C. Peterson, Bolyn Hubby, Matthew Coleman
http://dx.doi.org/10.1074/jbc.m117.784561 journal-article He, W. et al., 2017. Cell-free production of a functional oligomeric form of aChlamydiamajor outer-membrane protein (MOMP) for vaccine development. Journal of Biological Chemistry, 292(36), pp.15121–15132. Available at: …
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Development of a ceramic injection molding process for liquid jet nozzles to be applied for X-ray free-electron lasers
24 Jul 2017 | BioXFEL Publications | Contributor(s): Volker Piotter, Alexander Klein, Klaus Plewa, Kenneth R. Beyerlein, Henry Chapman, Saša Bajt
http://dx.doi.org/10.1007/s00542-017-3493-7 journal-article Piotter, V. et al., 2017. Development of a ceramic injection molding process for liquid jet nozzles to be applied for X-ray free-electron lasers. Microsystem Technologies. Available at: http://dx.doi.org/10.1007/s00542-017-3493-7.
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A Bright Future for Serial Femtosecond Crystallography with XFELs
18 Jul 2017 | BioXFEL Publications | Contributor(s): Linda C. Johansson, Benjamin Stauch, Andrii Ishchenko, Vadim Cherezov
http://dx.doi.org/10.1016/j.tibs.2017.06.007 journal-article Johansson, L.C. et al., 2017. A Bright Future for Serial Femtosecond Crystallography with XFELs. Trends in Biochemical Sciences. Available at: http://dx.doi.org/10.1016/j.tibs.2017.06.007.
Linda C. Johansson and Benjamin Stauch and …
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Single-shot determination of focused FEL wave fields using iterative phase retrieval
18 Jul 2017 | BioXFEL Publications | Contributor(s): Masoud Mehrjoo, Klaus Giewekemeyer, Patrik Vagovič, Stephan Stern, Richard Bean, Marc Messerschmidt, Barbara Keitel, Elke Plönjes, Marion Kuhlmann, Tobias Mey, Evgeny A. Schneidmiller, Mikhail V. Yurkov, Torsten Limberg, Adrian P. Mancuso
http://dx.doi.org/10.1364/oe.25.017892 journal-article Mehrjoo, M. et al., 2017. Single-shot determination of focused FEL wave fields using iterative phase retrieval. Optics Express, 25(15), p.17892. Available at: http://dx.doi.org/10.1364/oe.25.017892.
Masoud Mehrjoo and Klaus Giewekemeyer and …
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Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody
17 Jul 2017 | BioXFEL Publications | Contributor(s): Andrii Ishchenko, Daniel Wacker, Mili Kapoor, Ai Zhang, Gye Won Han, Shibom Basu, Nilkanth N Patel, Marc Messerschmidt, Uwe Weierstall, Wei Liu, Vsevolod Katritch, Bryan L. Roth, Raymond C. Stevens, Vadim Cherezov
http://dx.doi.org/10.1073/pnas.1700891114 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Ishchenko, A. et al., 2017. Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody. Proceedings of the National Academy of Sciences, …
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Crystal structure of CO-bound cytochrome
c
oxidase determined by serial femtosecond X-ray crystallography at room temperature
11 Jul 2017 | BioXFEL Publications | Contributor(s): Izumi Ishigami, Nadia Zatsepin, Masahide Hikita, Chelsie E Conrad, Garrett Charles Nelson, Jesse David Coe, Shibom Basu, Thomas D. Grant, Matthew H. Seaberg, Raymond G. Sierra, Mark S. Hunter, Petra Fromme, Raimund Fromme, Syun-Ru Yeh, Denis L. Rousseau
http://dx.doi.org/10.1073/pnas.1705628114 Cytochromecoxidase (CcO), the terminal enzyme in the electron transfer chain, translocates protons across the inner mitochondrial membrane by harnessing the free energy generated by the reduction of oxygen to water. Several redox-coupled proton …
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FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images
07 Jul 2017 | BioXFEL Publications | Contributor(s): Kenneth R. Beyerlein, Thomas A. White, Oleksandr Yefanov, Cornelius Gati, Ivan G. Kazantsev, Nicolai Fog-Gade Nielsen, Peter M. Larsen, Henry Chapman, Søren Schmidt
http://dx.doi.org/10.1107/s1600576717007506 A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented. The algorithm, FELIX, utilizes a generalized parametrization of the Rodrigues–Frank space, in which …
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Continuous diffraction of molecules and disordered molecular crystals
07 Jul 2017 | BioXFEL Publications | Contributor(s): Henry Chapman, Oleksandr M. Yefanov, Kartik Ayyer, Thomas A. White, Anton Barty, Andrew Morgan, Valerio Mariani, Dominik Oberthuer, Kanupriya Pande
http://dx.doi.org/10.1107/s160057671700749x The intensities of far-field diffraction patterns of orientationally aligned molecules obey Wilson statistics, whether those molecules are in isolation (giving rise to a continuous diffraction pattern) or arranged in a crystal (giving rise to Bragg …
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Watching proteins function with time-resolved x-ray crystallography
03 Jul 2017 | BioXFEL Publications | Contributor(s): Vukica Šrajer, Marius Schmidt
http://dx.doi.org/10.1088/1361-6463/aa7d32 journal-article Šrajer, V. & Schmidt, M., 2017. Watching proteins function with time-resolved x-ray crystallography. Journal of Physics D: Applied Physics, 50(37), p.373001. Available at: http://dx.doi.org/10.1088/1361-6463/aa7d32.
Vukica Šrajer and …
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How Cubic Can Ice Be?
28 Jun 2017 | BioXFEL Publications | Contributor(s): Andrew J. Amaya, Harshad Pathak, Viraj P. Modak, Hartawan Laksmono, N. Duane Loh, Jonas A. Sellberg, Raymond G. Sierra, Trevor A. McQueen, Matt J. Hayes, Garth J. Williams, Marc Messerschmidt, Sébastien Boutet, Michael J. Bogan, Anders Nilsson, Claudiu A. Stan, Barbara E. Wyslouzil
http://dx.doi.org/10.1021/acs.jpclett.7b01142 journal-article Amaya, A.J. et al., 2017. How Cubic Can Ice Be? The Journal of Physical Chemistry Letters, pp.3216–3222. Available at: http://dx.doi.org/10.1021/acs.jpclett.7b01142.
Andrew J. Amaya and Harshad Pathak and Viraj P. Modak and Hartawan …
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Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
27 Jun 2017 | BioXFEL Publications | Contributor(s): Hemanth K.N. Reddy, Chun Hong Yoon, Andrew Aquila, Salah Awel, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Sergey Bobkov, Maximilian Bucher, Gabriella A. Carini, Sebastian Carron, Henry Chapman, Benedikt Daurer, Hasan Demirci, Tomas Ekeberg, Petra Fromme, Janos Hajdu, Max Felix Hanke, Philip Hart, Brenda Hogue, Ahmad Hosseinizadeh, Yoonhee Kim, Richard Kirian, Ruslan P. Kurta, Daniel S.D. Larsson, N. Duane Loh, Filipe R.N.C. Maia, Adrian P. Mancuso, Kerstin Mühlig, Anna Munke, Daewoong Nam, Carl Nettelblad, Abbas Ourmazd, Max Rose, Peter Schwander, Marvin Seibert, Jonas A. Sellberg, Changyong Song, John Spence, Martin Svenda, Gijs Van Der Schot, Ivan A. Vartanyants, Garth J. Williams, P. Lourdu Xavier
http://dx.doi.org/10.1038/sdata.2017.79 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Reddy, H.K.N. et al., 2017. Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. Scientific Data, 4, p.170079. Available at: …
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Algorithm for Reconstruction of 3D Images of Nanorice Particles from Diffraction Patterns of Two Particles in Independent Random Orientations with an X-ray Laser
23 Jun 2017 | BioXFEL Publications | Contributor(s): Sung Soon Kim, Sandi Wibowo, Dilano Saldin
http://dx.doi.org/10.3390/app7070646 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Kim, S., Wibowo, S. & Saldin, D., 2017. Algorithm for Reconstruction of 3D Images of Nanorice Particles from Diffraction Patterns of Two Particles in Independent Random Orientations with an X-ray …
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Critical Role of Water Molecules in Proton Translocation by the Membrane-Bound Transhydrogenase
22 Jun 2017 | BioXFEL Publications | Contributor(s): Pius S. Padayatti, Josephine H. Leung, Paween Mahinthichaichan, Emad Tajkhorshid, Andrii Ishchenko, Vadim Cherezov, S. Michael Soltis, J. Baz Jackson, C. David Stout, Robert B. Gennis, Qinghai Zhang
http://dx.doi.org/10.1016/j.str.2017.05.022 journal-article Padayatti, P.S. et al., 2017. Critical Role of Water Molecules in Proton Translocation by the Membrane-Bound Transhydrogenase. Structure, 25(7), pp.1111–1119.e3. Available at: http://dx.doi.org/10.1016/j.str.2017.05.022.
Pius S. …
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Non-thermal damage to lead tungstate induced by intense short-wavelength laser radiation (Conference Presentation)
21 Jun 2017 | BioXFEL Publications | Contributor(s): Vojtech Vozda, Pavel Boháček, Tomáš Burian, Jaromir Chalupský, Vera Hájková, Libor Juha, Ludek Vyšín, Jérôme Gaudin, Philip A. Heimann, Stefan Hau-Riege, Marek Jurek, Dorota Klinger, Jacek Krzywinski, Marc Messerschmidt, Stefan P. Moeller, Robert Nagler, Jerzy B. Pelka, Michael Rowen, William F. Schlotter, Michele L. Swiggers, Harald Sinn, Ryszard Sobierajski, Kai Tiedtke, Sven Toleikis, Thomas Tschentscher, Joshua J. Turner, Hubertus Wabnitz, Art J. Nelson, Maria V. Kozlova, Sam M. Vinko, Thomas Whitcher, Thomas Dzelzainis, Oldrich Renner, Karel Saksl, Roland R. Fäustlin, Ali R. Khorsand, Marta Fajardo, Bianca S. Iwan, Jakob Andreasson, Janos Hajdu, Nicusor Timneanu, Justin S. Wark, David Riley, Richard W. Lee, Mitsuru Nagasono, Makina Yabashi
http://dx.doi.org/10.1117/12.2270944 proceedings-article Vozda, V. et al., 2017. Non-thermal damage to lead tungstate induced by intense short-wavelength laser radiation (Conference Presentation) L. Juha, S. Bajt, & R. Soufli, eds. Damage to VUV, EUV, and X-ray Optics VI. Available at: …
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Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography
21 Jun 2017 | BioXFEL Publications | Contributor(s): Dominik Oberthuer, Juraj Knoška, Max Oliver Wiedorn, Kenneth R. Beyerlein, David Bushnell, Elena G. Kovaleva, Michael Heymann, Lars Gumprecht, Richard Kirian, Anton Barty, Valerio Mariani, Aleksandra Tolstikova, Luigi Adriano, Salah Awel, Miriam Barthelmess, Katerina Dörner, P. Lourdu Xavier, Oleksandr Yefanov, Daniel R. James, Garrett Charles Nelson, Dingjie Wang, George Douglas Calvey, Yujie Chen, Andrea Schmidt, Michael Szczepek, Stefan Frielingsdorf, Oliver Lenz, Edward Snell, Philip J. Robinson, Božidar Šarler, Grega Belšak, Marjan Maček, Fabian Wilde, Andrew Aquila, Sébastien Boutet, Mengning Liang, Mark S. Hunter, Patrick Scheerer, John D. Lipscomb, Uwe Weierstall, Roger Kornberg, John Spence, Lois Pollack, Henry Chapman, Saša Bajt
http://dx.doi.org/10.1038/srep46846 journal-article Oberthuer, D. et al., 2017. Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7, p.46846. Available at: http://dx.doi.org/10.1038/srep46846.
Dominik Oberthuer and Juraj …
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Structure of CC Chemokine Receptor 5 with a Potent Chemokine Antagonist Reveals Mechanisms of Chemokine Recognition and Molecular Mimicry by HIV
21 Jun 2017 | BioXFEL Publications | Contributor(s): Yi Zheng, Gye Won Han, Ruben Abagyan, Beili Wu, Raymond C. Stevens, Vadim Cherezov, Irina Kufareva, Tracy M. Handel
http://dx.doi.org/10.1016/j.immuni.2017.05.002 journal-article Zheng, Y. et al., 2017. Structure of CC Chemokine Receptor 5 with a Potent Chemokine Antagonist Reveals Mechanisms of Chemokine Recognition and Molecular Mimicry by HIV. Immunity, 46(6), pp.1005–1017.e5. Available at: …
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Synthesis and biochemical characterization of EGF receptor in a water-soluble membrane model system
06 Jun 2017 | BioXFEL Publications | Contributor(s): Tiffany M. Scharadin, Wei He, Yianni Yiannakou, Alexey A. Tomilov, Matthew Saldana, Gino A. Cortopassi, Kermit L. Carraway, Matthew Coleman, Paul T. Henderson
http://dx.doi.org/10.1371/journal.pone.0177761 journal-article Scharadin, T.M. et al., 2017. Synthesis and biochemical characterization of EGF receptor in a water-soluble membrane model system L. Buday, ed. PLOS ONE, 12(6), p.e0177761. Available at: …
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Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser
02 Jun 2017 | BioXFEL Publications | Contributor(s): David Popp, N. Duane Loh, Habiba Zorgati, Umesh Ghoshdastider, Lu Ting Liow, Magdalena I. Ivanova, Mårten Larsson, Daniel P. DePonte, Richard Bean, Kenneth R. Beyerlein, Cornelius Gati, Dominik Oberthuer, David Arnlund, Gisela Brändén, Peter Berntsen, Duilio Cascio, Leonard M. G. Chavas, Joe Chen, Ke Ding, Holger Fleckenstein, Lars Gumprecht, Rajiv Harimoorthy, Estelle Mossou, Michael R. Sawaya, Aaron S. Brewster, Johan Hattne, Nicholas K. Sauter, Marvin Seibert, Carolin Seuring, Francesco Stellato, Thomas Tilp, David S. Eisenberg, Marc Messerschmidt, Garth J. Williams, Jason E. Koglin, Lee Makowski, Rick P. Millane, Trevor Forsyth, Sébastien Boutet, Thomas A. White, Anton Barty, Henry Chapman, Swaine L. Chen, Mengning Liang, Richard Neutze, Robert C. Robinson
http://dx.doi.org/10.1002/cm.21378 journal-article Popp, D. et al., 2017. Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser. Cytoskeleton. Available at: http://dx.doi.org/10.1002/cm.21378.
David Popp and N. Duane Loh and Habiba Zorgati and Umesh …
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Outrunning damage: Electrons vs X-rays—timescales and mechanisms
01 Jun 2017 | BioXFEL Publications | Contributor(s): John Spence
http://dx.doi.org/10.1063/1.4984606 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Spence, J.C.H., 2017. Outrunning damage: Electrons vs X-rays—timescales and mechanisms. Structural Dynamics, 4(4), p.044027. Available at: http://dx.doi.org/10.1063/1.4984606.
John C. …
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Crystallization of Membrane Proteins: An Overview
01 Jun 2017 | BioXFEL Publications | Contributor(s): Andrii Ishchenko, Enrique E. Abola, Vadim Cherezov
http://dx.doi.org/10.1007/978-1-4939-7000-1_5 book-chapter Ishchenko, A., Abola, E.E. & Cherezov, V., 2017. Crystallization of Membrane Proteins: An Overview. Protein Crystallography, pp.117–141. Available at: http://dx.doi.org/10.1007/978-1-4939-7000-1_5.
Andrii Ishchenko and Enrique E. Abola …
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Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources
01 Jun 2017 | BioXFEL Publications | Contributor(s): Marius Schmidt
http://dx.doi.org/10.1007/978-1-4939-7000-1_11 book-chapter NSF-STC Biology with X-ray Lasers (NSF-1231306) Schmidt, M., 2017. Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources. Protein Crystallography, pp.273–294. Available at: http://dx.doi.org/10.1007/978-1-4939-7000-1_11.
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Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation
24 May 2017 | BioXFEL Publications | Contributor(s): Jose M. Martin-Garcia, Chelsie E Conrad, Garrett Charles Nelson, Natasha Stander, Nadia Zatsepin, James D Zook, Lan Zhu, James Geiger, Eugene Chun, David Kissick, Mark C. Hilgart, Craig Ogata, Andrii Ishchenko, Nirupa Nagaratnam, Shatabdi Roy-Chowdhury, Jesse David Coe, GANESH SUBRAMANIAN, Alexander Schaffer, Daniel James, Gihan Ketwala, Nagarajan Venugopalan, Shenglan Xu, Stephen Corcoran, Dale Ferguson, Uwe Weierstall, John Spence, Vadim Cherezov, Petra Fromme, Robert F. Fischetti, Wei Liu
http://dx.doi.org/10.1107/s205225251700570x Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render …
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Structure of the full-length glucagon class B G-protein-coupled receptor
17 May 2017 | BioXFEL Publications | Contributor(s): Haonan Zhang, Anna Qiao, Dehua Yang, Linlin Yang, Antao Dai, Chris De Graaf, Steffen Reedtz-Runge, Venkatasubramanian Dharmarajan, Hui Zhang, Gye Won Han, Thomas D. Grant, Raymond G. Sierra, Uwe Weierstall, Garrett Charles Nelson, Wei Liu, Yanhong Wu, Limin Ma, Xiaoqing Cai, Guangyao Lin, Xiaoai Wu, Zhi Geng, Yuhui Dong, Gaojie Song, Patrick R. Griffin, Jesper Lau, Vadim Cherezov, Huaiyu Yang, Michael A. Hanson, Raymond C. Stevens, Qiang Zhao, Hualiang Jiang, Ming-Wei Wang, Beili Wu
http://dx.doi.org/10.1038/nature22363 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Zhang, Haonan et al., 2017. Structure of the full-length glucagon class B G-protein-coupled receptor. Nature. Available at: http://dx.doi.org/10.1038/nature22363.
Haonan Zhang and Anna Qiao and …
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Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand
17 May 2017 | BioXFEL Publications | Contributor(s): Xianjun Zhang, Fei Zhao, Yiran Wu, Jun Yang, Gye Won Han, Suwen Zhao, Andrii Ishchenko, Lintao Ye, Xi Lin, Kang Ding, Venkatasubramanian Dharmarajan, Patrick R. Griffin, Cornelius Gati, Garrett Charles Nelson, Mark S. Hunter, Michael A. Hanson, Vadim Cherezov, Raymond C. Stevens, Wenfu Tan, Houchao Tao, Fei Xu
http://dx.doi.org/10.1038/ncomms15383 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Zhang, X. et al., 2017. Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand. Nature Communications, 8, p.15383. Available at: …
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Peripheral Blood Biomarkers Associated With Toxicity and Treatment Characteristics After 131 I- Metaiodobenzylguanidine Therapy in Patients With Neuroblastoma
15 May 2017 | BioXFEL Publications | Contributor(s): Kevin Campbell, Erin E. Karski, Aleksandra Olow, David A. Edmondson, Ayano C. Kohlgruber, Matthew Coleman, Daphne A. Haas-Kogan, Katherine K. Matthay, Steven G. DuBois
http://dx.doi.org/10.1016/j.ijrobp.2017.05.008 journal-article Campbell, K. et al., 2017. Peripheral Blood Biomarkers Associated With Toxicity and Treatment Characteristics After 131 I- Metaiodobenzylguanidine Therapy in Patients With Neuroblastoma. International Journal of Radiation …
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Chemically Stable Lipids for Membrane Protein Crystallization
12 May 2017 | BioXFEL Publications | Contributor(s): Andrii Ishchenko, Lingling Peng, Egor Zinovev, Alexey Vlasov, Sung Chang Lee, Alexander Kuklin, Alexey Mishin, Valentin Borshchevskiy, Qinghai Zhang, Vadim Cherezov
http://dx.doi.org/10.1021/acs.cgd.7b00458 journal-article Ishchenko, A. et al., 2017. Chemically Stable Lipids for Membrane Protein Crystallization. Crystal Growth & Design. Available at: http://dx.doi.org/10.1021/acs.cgd.7b00458.
Andrii Ishchenko and Lingling Peng and Egor Zinovev and Alexey …
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XFELs for structure and dynamics in biology
10 May 2017 | BioXFEL Publications | Contributor(s): J. C. H. Spence
http://dx.doi.org/10.1107/s2052252517005760 The development and application of the free-electron X-ray laser (XFEL) to structure and dynamics in biology since its inception in 2009 are reviewed. The research opportunities which result from the ability to outrun most radiation-damage effects are …
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Proposal Writing Workshop Agenda
08 May 2017 | Downloads | Contributor(s): Bill Bauer
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Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli
25 Apr 2017 | BioXFEL Publications | Contributor(s): Mimi C. Yung, Feliza A. Bourguet, Timothy S. Carpenter, Matthew Coleman
http://dx.doi.org/10.1186/s12934-017-0685-x journal-article Yung, M.C. et al., 2017. Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli. Microbial Cell Factories, 16(1). Available at: …
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Laser control over the ultrafast Coulomb explosion of
N22+
after Auger decay: A quantum-dynamics investigation
24 Apr 2017 | BioXFEL Publications | Contributor(s): Athiya Mahmud Hanna, Oriol Vendrell, Abbas Ourmazd, Robin Santra
http://dx.doi.org/10.1103/physreva.95.043419 journal-article Hanna, A.M. et al., 2017. Laser control over the ultrafast Coulomb explosion of N22+ after Auger decay: A quantum-dynamics investigation. Physical Review A, 95(4). Available at: http://dx.doi.org/10.1103/physreva.95.043419.
Athiya …
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X-Ray Lasers in Biology
24 Apr 2017 | BioXFEL Publications | Contributor(s): John Spence
http://dx.doi.org/10.1016/bs.aiep.2017.01.008 book-chapter NSF-STC Biology with X-ray Lasers (NSF-1231306) Spence, J.C.H., 2017. X-Ray Lasers in Biology. Advances in Imaging and Electron Physics, pp.103–152. Available at: http://dx.doi.org/10.1016/bs.aiep.2017.01.008.
John C.H. Spence
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Split-Second Reactions
24 Apr 2017 | BioXFEL Publications | Contributor(s): Petra Fromme, John Spence
http://dx.doi.org/10.1038/scientificamerican0517-62 journal-article Fromme, P. & Spence, J.C.H., 2017. Split-Second Reactions. Scientific American, 316(5), pp.62–67. Available at: http://dx.doi.org/10.1038/scientificamerican0517-62.
Petra Fromme and John C. H. Spence
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A multi-model approach to assessing local and global cryo-EM map quality
20 Apr 2017 | BioXFEL Publications | Contributor(s): Mark A Herzik, James Fraser, Gabriel C Lander
http://dx.doi.org/10.1101/128561 There does not currently exist a standardized indicator of how well a cryo-EM-derived model represents the density from which it was generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models …
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Flexibility and Design: Conformational Heterogeneity along the Evolutionary Trajectory of a Redesigned Ubiquitin
13 Apr 2017 | BioXFEL Publications | Contributor(s): Justin T. Biel, Michael Thompson, Christian N. Cunningham, Jacob E. Corn, James Fraser
http://dx.doi.org/10.1016/j.str.2017.03.009 journal-article Biel, J.T. et al., 2017. Flexibility and Design: Conformational Heterogeneity along the Evolutionary Trajectory of a Redesigned Ubiquitin. Structure, 25(5), pp.739–749.e3. Available at: http://dx.doi.org/10.1016/j.str.2017.03.009.
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Diffraction data of core-shell nanoparticles from an X-ray free electron laser
11 Apr 2017 | BioXFEL Publications | Contributor(s): Xuanxuan Li, Chun-Ya Chiu, Hsiang-Ju Wang, Stephan Kassemeyer, Sabine Botha, Robert L. Shoeman, Robert M Lawrence, Christopher Kupitz, Richard Kirian, Daniel James, Dingjie Wang, Garrett Charles Nelson, Marc Messerschmidt, Sébastien Boutet, Garth J. Williams, Elisabeth Hartmann, Aliakbar Jafarpour, Lutz M. Foucar, Anton Barty, Henry Chapman, Mengning Liang, Andreas Menzel, Fenglin Wang, Shibom Basu, Raimund Fromme, R. Bruce Doak, Petra Fromme, Uwe Weierstall, Michael H. Huang, John Spence, Ilme Schlichting, Brenda Hogue, Haiguang Liu
http://dx.doi.org/10.1038/sdata.2017.48 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Li, X. et al., 2017. Diffraction data of core-shell nanoparticles from an X-ray free electron laser. Scientific Data, 4, p.170048. Available at: http://dx.doi.org/10.1038/sdata.2017.48.
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Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
07 Apr 2017 | BioXFEL Publications | Contributor(s): Benedikt J. Daurer, Kenta Okamoto, Johan Bielecki, Filipe R. N. C. Maia, Kerstin Mühlig, M. Marvin Seibert, Max F. Hantke, Carl Nettelblad, W. Henry Benner, Martin Svenda, Nicuşor Tîmneanu, Tomas Ekeberg, N. Duane Loh, Alberto Pietrini, Alessandro Zani, Asawari D. Rath, Daniel Westphal, Richard Kirian, Salah Awel, Max Oliver Wiedorn, Gijs Van Der Schot, Gunilla H. Carlsson, Dirk Hasse, Jonas A. Sellberg, Anton Barty, Jakob Andreasson, Sébastien Boutet, Garth Williams, Jason Koglin, Inger Andersson, Janos Hajdu, Daniel S. D. Larsson
http://dx.doi.org/10.1107/s2052252517003591 This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. AerosolizedOmono River …
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Structural basis for selectivity and diversity in angiotensin II receptors
04 Apr 2017 | BioXFEL Publications | Contributor(s): Haitao Zhang, Gye Won Han, Alexander Batyuk, Andrii Ishchenko, Kate L. White, Nilkanth N Patel, Anastasiia Sadybekov, Beata Zamlynny, Michael T. Rudd, Kaspar Hollenstein, Alexandra Tolstikova, Thomas A. White, Mark S. Hunter, Uwe Weierstall, Wei Liu, Kerim Babaoglu, Eric L. Moore, Ryan D. Katz, Jennifer M. Shipman, Margarita Garcia-Calvo, Sujata Sharma, Payal Sheth, Stephen M. Soisson, Raymond C. Stevens, Vsevolod Katritch, Vadim Cherezov
http://dx.doi.org/10.1038/nature22035 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Zhang, H. et al., 2017. Structural basis for selectivity and diversity in angiotensin II receptors. Nature, 544(7650), pp.327–332. Available at: http://dx.doi.org/10.1038/nature22035.
Haitao …
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Chromatin-remodeling for transcription
17 Mar 2017 | BioXFEL Publications | Contributor(s): Yahli Lorch, Roger Kornberg
Abstract
The nucleosome serves as a general gene repressor, preventing all initiation of transcription except that which is brought about by specific positive regulatory mechanisms. The positive mechanisms begin with chromatin-remodeling by complexes that slide, disrupt, or otherwise …
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Double-flow focused liquid injector for efficient serial femtosecond crystallography
16 Mar 2017 | BioXFEL Publications | Contributor(s): Dominik Oberthuer, Juraj Knoška, Max Oliver Wiedorn, Kenneth R. Beyerlein, David Bushnell, Elena G. Kovaleva, Michael Heymann, Lars Gumprecht, Richard Kirian, Anton Barty, Valerio Mariani, Aleksandra Tolstikova, Luigi Adriano, Salah Awel, Miriam Barthelmess, Katerina Dörner, P. Lourdu Xavier, Oleksandr Yefanov, Daniel R. James, Garrett Charles Nelson, Dingjie Wang, George Douglas Calvey, Yujie Chen, Andrea Schmidt, Michael Szczepek, Stefan Frielingsdorf, Oliver Lenz, Edward Snell, Philip J. Robinson, Božidar Šarler, Grega Belšak, Marjan Maček, Fabian Wilde, Andrew Aquila, Sébastien Boutet, Mengning Liang, Mark S. Hunter, Patrick Scheerer, John D. Lipscomb, Uwe Weierstall, Roger Kornberg, John Spence, Lois Pollack, Henry Chapman, Saša Bajt
http://dx.doi.org/10.1038/srep44628 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Oberthuer, D. et al., 2017. Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7, p.44628. Available at: http://dx.doi.org/10.1038/srep44628.
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Moving in the Right Direction: Protein Vibrational Steering Function
14 Mar 2017 | BioXFEL Publications | Contributor(s): Katherine A. Niessen, Mengyang Xu, Alessandro Paciaroni, Andrea Orecchini, Edward Snell, Andrea Markelz
journal-article http://dx.doi.org/10.1016/j.bpj.2016.12.049 Niessen, K.A. et al., 2017. Moving in the Right Direction: Protein Vibrational Steering Function. Biophysical Journal, 112(5), pp.933–942. Available at: http://dx.doi.org/10.1016/j.bpj.2016.12.049.
Katherine A. Niessen and Mengyang Xu …
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Synthesis of two fluorescent GTPγS molecules and their biological relevance
10 Mar 2017 | BioXFEL Publications | Contributor(s): Denise J. Trans, Ruoli Bai, J. Bennet Addison, Ruiwu Liu, Ernest Hamel, Matthew Coleman, Paul T. Henderson
http://dx.doi.org/10.1080/15257770.2016.1231320 journal-article Trans, D.J. et al., 2017. Synthesis of two fluorescent GTPγS molecules and their biological relevance. Nucleosides, Nucleotides and Nucleic Acids, 36(6), pp.379–391. Available at: http://dx.doi.org/10.1080/15257770.2016.1231320.
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Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers
02 Mar 2017 | BioXFEL Publications | Contributor(s): Franklin D Fuller, Sheraz Gul, Ruchira Chatterjee, E Sethe Burgie, Iris Diane Young, Hugo Lebrette, Vivek Srinivas, Aaron S Brewster, Tara Michels-Clark, Jonathan Clinger, Babak Andi, Mohamed Ibrahim, Ernest Pastor, Casper De Lichtenberg, Rana Hussein, Christopher J Pollock, Miao Zhang, Claudiu A Stan, Thomas Kroll, Thomas Fransson, Clemens Weninger, Markus Kubin, Pierre Aller, Louise Lassalle, Philipp Bräuer, Mitchell Miller, Muhamed Amin, Sergey Koroidov, Christian G Roessler, Marc Allaire, Raymond G Sierra, Peter T Docker, James M Glownia, Silke Nelson, Jason E Koglin, Diling Zhu, Matthieu Chollet, Sanghoon Song, Henrik Lemke, Mengning Liang, Dimosthenis Sokaras, Roberto Alonso-Mori, Athina Zouni, Johannes Messinger, Uwe Bergmann, Amie K Boal, J Martin Bollinger, Carsten Krebs, Martin Högbom, George Phillips, Richard D Vierstra, Nicholas K Sauter, Allen M Orville, Jan Kern, Vittal K Yachandra, Junko Yano
journal-article http://dx.doi.org/10.1038/nmeth.4195 NSF-STC Biology with X-ray Lasers (NSF-1231306) Fuller, F.D. et al., 2017. Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. Nature Methods, 14(4), pp.443–449. Available at: …
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Ondrej Krivanek's early scientific research
01 Mar 2017 | BioXFEL Publications | Contributor(s): John Spence
http://dx.doi.org/10.1016/j.ultramic.2016.12.006 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Spence, J.C.H., 2017. Ondrej Krivanek’s early scientific research. Ultramicroscopy, 180, pp.8–13. Available at: http://dx.doi.org/10.1016/j.ultramic.2016.12.006.
John C.H. Spence
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Serial crystallography at synchrotrons and X-ray lasers
23 Feb 2017 | BioXFEL Publications | Contributor(s): Jörg Standfuss, John Spence
http://dx.doi.org/10.1107/s2052252517001877 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Standfuss, J. & Spence, J., 2017. Serial crystallography at synchrotrons and X-ray lasers. IUCrJ, 4(2), pp.100–101. Available at: http://dx.doi.org/10.1107/s2052252517001877.
Jörg …
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An Engineered TGF-β Monomer that Functions as a Dominant Negative to Block TGF-β Signaling
22 Feb 2017 | BioXFEL Publications | Contributor(s): Sun Kyung Kim, Lindsey Barron, Cynthia S. Hinck, Elyse M. Petrunak, Kristin E. Cano, Avinash Thangirala, Brian Iskra, Molly Brothers, Machell Vonberg, Belinda Leal, Blair Richter, Ravindra Kodali, Alexander B. Taylor, Shoucheng Du, Christopher Barnes, Traian Sulea, Guillermo Calero, P. John Hart, Matthew J. Hart, Borries Demeler, Andrew P. Hinck
journal-article http://dx.doi.org/10.1074/jbc.m116.768754 Kim, S.K. et al., 2017. An Engineered TGF-β Monomer that Functions as a Dominant Negative to Block TGF-β Signaling. Journal of Biological Chemistry, p.jbc.M116.768754. Available at: http://dx.doi.org/10.1074/jbc.m116.768754.
Sun Kyung …
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Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide
22 Feb 2017 | BioXFEL Publications | Contributor(s): Arpan Deb, William A. Johnson, Alexander P. Kline, Boston J. Scott, Lydia R. Meador, Dustin Srinivas, Jose M. Martin-Garcia, Katerina Dörner, Chad R. Borges, Rajeev Misra, Brenda Hogue, Petra Fromme, Tsafrir S. Mor
http://dx.doi.org/10.1371/journal.pone.0172529 journal-article Deb, A. et al., 2017. Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide M. Schindler, ed. PLOS ONE, 12(2), p.e0172529. Available at: …
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Mechanism of Human Apohemoglobin Unfolding
21 Feb 2017 | BioXFEL Publications | Contributor(s): Premila P. Samuel, William C. Ou, George Phillips, John S. Olson
http://dx.doi.org/10.1021/acs.biochem.6b01235 journal-article Samuel, P.P. et al., 2017. Mechanism of Human Apohemoglobin Unfolding. Biochemistry, 56(10), pp.1444–1459. Available at: http://dx.doi.org/10.1021/acs.biochem.6b01235.
Premila P. Samuel and William C. Ou and George N. Phillips and …
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A scalable lysyl hydroxylase 2 expression system and luciferase-based enzymatic activity assay
21 Feb 2017 | BioXFEL Publications | Contributor(s): Hou-Fu Guo, Eun Jeong Cho, Ashwini K. Devkota, Yulong Chen, William Russell, George Phillips, Mitsuo Yamauchi, Kevin N. Dalby, Jonathan M. Kurie
journal-article http://dx.doi.org/10.1016/j.abb.2017.02.003 Guo, H.-F. et al., 2017. A scalable lysyl hydroxylase 2 expression system and luciferase-based enzymatic activity assay. Archives of Biochemistry and Biophysics, 618, pp.45–51. Available at: http://dx.doi.org/10.1016/j.abb.2017.02.003.
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The impact of base stacking on the conformations and electrostatics of single-stranded DNA
17 Feb 2017 | BioXFEL Publications | Contributor(s): Alex Plumridge, Steve P. Meisburger, Kurt Andresen, Lois Pollack
http://dx.doi.org/10.1093/nar/gkx140 journal-article Plumridge, A. et al., 2017. The impact of base stacking on the conformations and electrostatics of single-stranded DNA. Nucleic Acids Research. Available at: http://dx.doi.org/10.1093/nar/gkx140.
Alex Plumridge and Steve P. Meisburger and Kurt …
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Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser
15 Feb 2017 | BioXFEL Publications | Contributor(s): Cornelius Gati, Dominik Oberthuer, Oleksandr Yefanov, Richard D. Bunker, Francesco Stellato, Elaine Chiu, Shin-Mei Yeh, Andrew Aquila, Shibom Basu, Richard Bean, Kenneth R. Beyerlein, Sabine Botha, Sébastien Boutet, Daniel P. DePonte, R. Bruce Doak, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Daniel R. James, Christopher Kupitz, Lukas Lomb, Marc Messerschmidt, Karol Nass, Kimberly Rendek, Robert L. Shoeman, Dingjie Wang, Uwe Weierstall, Thomas A. White, Garth J. Williams, Nadia Zatsepin, Petra Fromme, John Spence, Kenneth N. Goldie, Johannes A. Jehle, Peter Metcalf, Anton Barty, Henry Chapman
journal-article http://dx.doi.org/10.1073/pnas.1609243114 NSF-STC Biology with X-ray Lasers (NSF-1231306) Gati, C. et al., 2017. Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser. Proceedings of the National Academy of Sciences, …
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Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
13 Feb 2017 | BioXFEL Publications | Contributor(s): M Jason De La Cruz, Johan Hattne, Dan Shi, Paul Seidler, Jose Rodriguez, Francis E Reyes, Michael R Sawaya, Duilio Cascio, Simon C Weiss, Sun Kyung Kim, Cynthia S Hinck, Andrew P Hinck, Guillermo Calero, David Eisenberg, Tamir Gonen
journal-article http://dx.doi.org/10.1038/nmeth.4178 NSF-STC Biology with X-ray Lasers (NSF-1231306) De la Cruz, M.J. et al., 2017. Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. Nature Methods, 14(4), pp.399–402. Available at: …
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Structure and specificity of a permissive bacterial C-prenyltransferase
06 Feb 2017 | BioXFEL Publications | Contributor(s): Sherif I Elshahawi, Hongnan Cao, Khaled A Shaaban, Larissa V Ponomareva, Thangaiah Subramanian, Mark L Farman, H Peter Spielmann, George Phillips, Jon S Thorson, Shanteri Singh
journal-article http://dx.doi.org/10.1038/nchembio.2285 Elshahawi, S.I. et al., 2017. Structure and specificity of a permissive bacterial C-prenyltransferase. Nature Chemical Biology, 13(4), pp.366–368. Available at: http://dx.doi.org/10.1038/nchembio.2285.
Sherif I Elshahawi and Hongnan Cao …
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Retinal oxidative stress at the onset of diabetes determined by synchrotron FTIR widefield imaging: towards diabetes pathogenesis
06 Feb 2017 | BioXFEL Publications | Contributor(s): Ebrahim Aboualizadeh, Mahsa Ranji, Christine M. Sorenson, Reyhaneh Sepehr, Nader Sheibani, Carol J. Hirschmugl
A mechanistic understanding of oxidative damage in the retina and the significance of photoreceptors at the onset of diabetic retinopathy are reported. journal-article http://dx.doi.org/10.1039/c6an02603f Aboualizadeh, E. et al., 2017. Retinal oxidative stress at the onset of diabetes determined …
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Model Comparison to Identify Structural Heterogeneity in Protein X-Ray Crystallography
04 Feb 2017 | BioXFEL Publications | Contributor(s): Nathan S. Babcock, David A. Sivak, Daniel Keedy, James Fraser
http://dx.doi.org/10.1016/j.bpj.2016.11.2628 journal-article Babcock, N.S. et al., 2017. Model Comparison to Identify Structural Heterogeneity in Protein X-Ray Crystallography. Biophysical Journal, 112(3), p.486a. Available at: http://dx.doi.org/10.1016/j.bpj.2016.11.2628.
Nathan S. Babcock and …
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Role of the MT1
and MT2
melatonin receptors in mediating depressive- and anxiety-like behaviors in C3H/HeN mice
03 Feb 2017 | BioXFEL Publications | Contributor(s): J. Liu, S. J. Clough, M. L. Dubocovich
http://dx.doi.org/10.1111/gbb.12369 journal-article Liu, J., Clough, S.J. & Dubocovich, M.L., 2017. Role of the MT1
and MT2
melatonin receptors in mediating depressive- and anxiety-like behaviors in C3H/HeN mice. Genes, Brain and Behavior, 16(5), pp.546–553. Available at: …
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A short history of structure based research on the photocycle of photoactive yellow protein
31 Jan 2017 | BioXFEL Publications | Contributor(s): Marius Schmidt
journal-article http://dx.doi.org/10.1063/1.4974172 NSF-STC Biology with X-ray Lasers (NSF-1231306) Schmidt, M., 2017. A short history of structure based research on the photocycle of photoactive yellow protein. Structural Dynamics, 4(3), p.032201. Available at: …
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Chromatin potentiates transcription
30 Jan 2017 | BioXFEL Publications | Contributor(s): Shigeki Nagai, Ralph E. Davis, Pierre Jean Mattei, Kyle Patrick Eagen, Roger Kornberg
journal-article http://dx.doi.org/10.1073/pnas.1620312114 Nagai, S. et al., 2017. Chromatin potentiates transcription. Proceedings of the National Academy of Sciences, 114(7), pp.1536–1541. Available at: http://dx.doi.org/10.1073/pnas.1620312114.
Shigeki Nagai and Ralph E. Davis and Pierre Jean …
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Cytidine deaminase efficiency of the lentiviral viral restriction factor APOBEC3C correlates with dimerization
30 Jan 2017 | BioXFEL Publications | Contributor(s): Madison B. Adolph, Anjuman Ara, Yuqing Feng, Cristina J. Wittkopp, Michael Emerman, James Fraser, Linda Chelico
journal-article http://dx.doi.org/10.1093/nar/gkx066 Adolph, M.B. et al., 2017. Cytidine deaminase efficiency of the lentiviral viral restriction factor APOBEC3C correlates with dimerization. Nucleic Acids Research, 45(6), pp.3378–3394. Available at: http://dx.doi.org/10.1093/nar/gkx066.
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Spermine Condenses DNA, but Not RNA Duplexes
10 Jan 2017 | BioXFEL Publications | Contributor(s): Andrea Katz, Igor S. Tolokh, Suzette A. Pabit, Nathan Baker, Alexey V. Onufriev, Lois Pollack
http://dx.doi.org/10.1016/j.bpj.2016.11.018 journal-article Katz, A.M. et al., 2017. Spermine Condenses DNA, but Not RNA Duplexes. Biophysical Journal, 112(1), pp.22–30. Available at: http://dx.doi.org/10.1016/j.bpj.2016.11.018.
Andrea M. Katz and Igor S. Tolokh and Suzette A. Pabit and Nathan …
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OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation
09 Jan 2017 | BioXFEL Publications | Contributor(s): Ryan R. Hughes, Khaled A. Shaaban, Jianjun Zhang, Hongnan Cao, George Phillips, Jon S. Thorson
http://dx.doi.org/10.1002/cbic.201600676 journal-article Hughes, R.R. et al., 2017. OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation. ChemBioChem, 18(4), pp.363–367. Available at: http://dx.doi.org/10.1002/cbic.201600676.
Ryan R. Hughes and Khaled A. Shaaban and Jianjun …
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Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core
27 Dec 2016 | BioXFEL Publications | Contributor(s): Yujie Chen, Joshua M. Tokuda, Traci Topping, Steve P. Meisburger, Suzette A. Pabit, Lisa M. Gloss, Lois Pollack
http://dx.doi.org/10.1073/pnas.1611118114 journal-article Chen, Y. et al., 2016. Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proceedings of the National Academy of Sciences, 114(2), pp.334–339. Available at: …
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Carbamate Insecticides Target Human Melatonin Receptors
27 Dec 2016 | BioXFEL Publications | Contributor(s): Marina Popovska-Gorevski, Margarita L. Dubocovich, Rajendram V. Rajnarayanan
http://dx.doi.org/10.1021/acs.chemrestox.6b00301 journal-article Popovska-Gorevski, M., Dubocovich, M.L. & Rajnarayanan, R.V., 2017. Carbamate Insecticides Target Human Melatonin Receptors. Chemical Research in Toxicology, 30(2), pp.574–582. Available at: …
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Conformational variation of proteins at room temperature is not dominated by radiation damage
21 Dec 2016 | BioXFEL Publications | Contributor(s): Silvia Russi, Ana González, Lillian R. Kenner, Daniel A. Keedy, James Fraser, Henry Van Den Bedem
Protein crystallography data collection at synchrotrons is routinely carried out at cryogenic temperatures to mitigate radiation damage. Although damage still takes place at 100 K and below, the immobilization of free radicals increases the lifetime of the crystals by approximately 100-fold. …
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LCLS Beam-time Schedules
20 Dec 2016 | Downloads
All Schedules on LCLS Website
LCLS Run 15 Schedule (June 2017 - Nov 2017)LCLS Run 14 Schedule (Aug 2016 - Dec 2016)LCLS Run 13 Schedule (Mar 2016 - Aug 2016)LCLS Run 12 Schedule (Oct 2015 - Mar 2016)LCLS Run 11 Schedule (Mar 2015 - Aug 2015)LCLS Run 10 Schedule (Oct 2014 - Mar 2015)
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Structural enzymology using X-ray free electron lasers
15 Dec 2016 | BioXFEL Publications | Contributor(s): Christopher Kupitz, Jose L. Olmos, Mark Holl, Lee Tremblay, Kanupriya Pande, Suraj Pandey, Dominik Oberthür, Mark S. Hunter, Mengning Liang, Andrew Aquila, Jason Tenboer, George Douglas Calvey, Andrea Katz, Yujie Chen, Max Oliver Wiedorn, Juraj Knoska, Alke Meents, Valerio Majriani, Tyler Norwood, Ishwor Poudyal, Thomas Grant, Mitchell Miller, Weijun Xu, Aleksandra Tolstikova, Andrew Morgan, Markus Metz, Jose M. Martin-Garcia, James D Zook, Shatabdi Roy-Chowdhury, Jesse David Coe, Nirupa Nagaratnam, Domingo Meza, Raimund Fromme, Shibom Basu, Matthias Frank, Thomas White, Anton Barty, Sasa Bajt, Oleksandr Yefanov, Henry Chapman, Nadia Zatsepin, Garrett Charles Nelson, Uwe Weierstall, John Spence, Peter Schwander, Lois Pollack, Petra Fromme, Abbas Ourmazd, George Phillips, Marius Schmidt
http://dx.doi.org/10.1063/1.4972069 Christopher Kupitz and Jose L. Olmos and Mark Holl and Lee Tremblay and Kanupriya Pande and Suraj Pandey and Dominik Oberthür and Mark Hunter and Mengning Liang and Andrew Aquila and Jason Tenboer and George Calvey and Andrea Katz and Yujie Chen and Max O. …
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Visualizing single-stranded nucleic acids in solution
13 Dec 2016 | BioXFEL Publications | Contributor(s): Alex Plumridge, Steve P. Meisburger, Lois Pollack
http://dx.doi.org/10.1093/nar/gkw1297 journal-article Plumridge, A., Meisburger, S.P. & Pollack, L., 2016. Visualizing single-stranded nucleic acids in solution. Nucleic Acids Research, p.gkw1297. Available at: http://dx.doi.org/10.1093/nar/gkw1297.
Alex Plumridge and Steve P. Meisburger and …
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Continuous Separation of DNA Molecules by Size Using Insulator-Based Dielectrophoresis
12 Dec 2016 | BioXFEL Publications | Contributor(s): Paul V. Jones, Gabriel L. Salmon, Alexandra Ros
journal-article http://dx.doi.org/10.1021/acs.analchem.6b03369 Jones, P.V., Salmon, G.L. & Ros, A., 2017. Continuous Separation of DNA Molecules by Size Using Insulator-Based Dielectrophoresis. Analytical Chemistry, 89(3), pp.1531–1539. Available at: …
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Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists
06 Dec 2016 | BioXFEL Publications | Contributor(s): Yi Zheng, Ling Qin, Natalia V. Ortiz Zacarías, Henk De Vries, Gye Won Han, Martin Gustavsson, Marta Dabros, Chunxia Zhao, Robert J. Cherney, Percy Carter, Dean Stamos, Ruben Abagyan, Vadim Cherezov, Raymond C. Stevens, Adriaan P. IJzerman, Laura H. Heitman, Andrew Tebben, Irina Kufareva, Tracy M. Handel
http://dx.doi.org/10.1038/nature20605 journal-article Zheng, Y. et al., 2016. Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists. Nature, 540(7633), pp.458–461. Available at: http://dx.doi.org/10.1038/nature20605.
Yi Zheng and Ling Qin and Natalia V. Ortiz …
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Lecture Series
01 Dec 2016 | Teaching Materials
BioXFEL Lecture Series videos are kept here.
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Journal Club Seminars
01 Dec 2016 | Teaching Materials
Videos of all past Journal Clubs
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Editorial overview: Biophysical and molecular biological methods
28 Nov 2016 | BioXFEL Publications | Contributor(s): Petra Fromme, Andrej Sali
http://dx.doi.org/10.1016/j.sbi.2016.11.015 journal-article Fromme, P. & Sali, A., 2016. Editorial overview: Biophysical and molecular biological methods. Current Opinion in Structural Biology, 40, pp.ix–xi. Available at: http://dx.doi.org/10.1016/j.sbi.2016.11.015.
Petra Fromme and Andrej Sali
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Structure of photosystem II and substrate binding at room temperature
18 Nov 2016 | BioXFEL Publications | Contributor(s): Iris Diane Young, Mohamed Ibrahim, Ruchira Chatterjee, Sheraz Gul, Franklin D. Fuller, Sergey Koroidov, Aaron S. Brewster, Rosalie Tran, Roberto Alonso-Mori, Thomas Kroll, Tara Michels-Clark, Hartawan Laksmono, Raymond G. Sierra, Claudiu A. Stan, Rana Hussein, Miao Zhang, Lacey Douthit, Markus Kubin, Casper De Lichtenberg, Long Vo Pham, Håkan Nilsson, Mun Hon Cheah, Dmitriy Shevela, Claudio Saracini, Mackenzie A. Bean, Ina Seuffert, Dimosthenis Sokaras, Tsu-Chien Weng, Ernest Pastor, Clemens Weninger, Thomas Fransson, Louise Lassalle, Philipp Bräuer, Pierre Aller, Peter T. Docker, Babak Andi, Allen M. Orville, James M. Glownia, Silke Nelson, Marcin Sikorski, Diling Zhu, Mark S. Hunter, Thomas J. Lane, Andy Aquila, Jason E. Koglin, Joseph Robinson, Mengning Liang, Sébastien Boutet, Artem Y. Lyubimov, Monarin Uervirojnangkoorn, Nigel W. Moriarty, Dorothee Liebschner, Pavel V. Afonine, David G. Waterman, Gwyndaf Evans, Philippe Wernet, Holger Dobbek, William I. Weis, Axel T. Brunger, Petrus H. Zwart, Paul D. Adams, Athina Zouni, Johannes Messinger, Uwe Bergmann, Nicholas K. Sauter, Jan Kern, Vittal K. Yachandra, Junko Yano
http://dx.doi.org/10.1038/nature20161 Iris D. Young and Mohamed Ibrahim and Ruchira Chatterjee and Sheraz Gul and Franklin D. Fuller and Sergey Koroidov and Aaron S. Brewster and Rosalie Tran and Roberto Alonso-Mori and Thomas Kroll and Tara Michels-Clark and Hartawan Laksmono and Raymond G. Sierra …
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Simulations of single-pulse Laue diffraction from proteins with radiation from synchrotron and XFEL sources
15 Nov 2016 | BioXFEL Publications | Contributor(s): Victoria Kabanova, Marius Schmidt, Friedrich Schotte, Marco Cammarata, Michael Wulff
http://dx.doi.org/10.1107/s2053273316097898 journal-article Kabanova, V. et al., 2016. Simulations of single-pulse Laue diffraction from proteins with radiation from synchrotron and XFEL sources. Acta Crystallographica Section A Foundations and Advances, 72(a1), pp.s142–s142. Available at: …
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Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
15 Nov 2016 | BioXFEL Publications | Contributor(s): Przemyslaw Nogly, Valerie Panneels, Garrett Charles Nelson, Cornelius Gati, Tetsunari Kimura, Christopher Milne, Despina Milathianaki, Minoru Kubo, Wenting Wu, Chelsie E Conrad, Jesse David Coe, Richard Bean, Yun Zhao, Petra Bath, Robert Dods, Rajiv Harimoorthy, Kenneth Beyerlein, Jan Rheinberger, Daniel James, Daniel DePonte, Chufeng Li, Leonardo Sala, Garth Williams, Mark S. Hunter, Jason E. Koglin, Peter Berntsen, Eriko Nango, So Iwata, Henry Chapman, Petra Fromme, Matthias Frank, Rafael Abela, Sébastien Boutet, Anton Barty, Thomas A. White, Uwe Weierstall, John Spence, Richard Neutze, Gebhard F.X. Schertler, Jörg Standfuss
http://dx.doi.org/10.1107/s2053273316099368 Przemyslaw Nogly and Valerie Panneels and Garrett Nelson and Cornelius Gati and Tetsunari Kimura and Christopher Milne and Despina Milathianaki and Minoru Kubo and Wenting Wu and Chelsie Conrad and Jesse Coe and Richard Bean and Yun Zhao and Petra Bath …
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The use of haptic interfaces and web services in crystallography: an application for a `screen to beam' interface
11 Nov 2016 | BioXFEL Publications | Contributor(s): Andrew E. Bruno, Alexei S. Soares, Robin L. Owen, Edward Snell
http://dx.doi.org/10.1107/s160057671601431x Haptic interfaces have become common in consumer electronics. They enable easy interaction and information entry without the use of a mouse or keyboard. The work presented here illustrates the application of a haptic interface to crystallization screening …
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Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography
11 Nov 2016 | BioXFEL Publications | Contributor(s): J. R. Stagno, Y. Liu, Y. R. Bhandari, C. E. Conrad, S. Panja, M. Swain, L. Fan, G. Nelson, C. Li, D. R. Wendel, T. A. White, J. D. Coe, M. O. Wiedorn, J. Knoska, D. Oberthuer, R. A. Tuckey, P. Yu, M. Dyba, S. G. Tarasov, U. Weierstall, T. D. Grant, C. D. Schwieters, J. Zhang, A. R. Ferré-D’Amaré, P. Fromme, D. E. Draper, M. Liang, M. S. Hunter, S. Boutet, K. Tan, X. Zuo, X. Ji, A. Barty, N. A. Zatsepin, H. N. Chapman, J. C. H. Spence, S. A. Woodson, Y.-X. Wang
http://dx.doi.org/10.1038/nature20599 J. R. Stagno and Y. Liu and Y. R. Bhandari and C. E. Conrad and S. Panja and M. Swain and L. Fan and G. Nelson and C. Li and D. R. Wendel and T. A. White and J. D. Coe and M. O. Wiedorn and J. Knoska and D. Oberthuer and R. A. Tuckey and P. Yu and M. Dyba and …
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Development of a photophoretic optical guide for femtosecond x-ray diffractive imaging of aerosolized nanoparticles
(Conference Presentation)
10 Nov 2016 | BioXFEL Publications | Contributor(s): Richard Kirian, Salah Awel, Max Oliver Wiedorn, Daniel Horke, Nils Roth, Niko O. Eckerskorn, Jochen Küpper, Henry Chapman, Andrei V. Rode
http://dx.doi.org/10.1117/12.2239171 Richard A. Kirian and Salah Awel and Max Wiedorn and Daniel Horke and Nils Roth and Niko O. Eckerskorn and Jochen Küpper and Henry N. Chapman and Andrei V. Rode Kirian, R.A. et al., 2016. Development of a photophoretic optical guide for femtosecond x-ray …
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4th BioXFEL Conference
04 Nov 2016 | Downloads
…
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Selenium single-wavelength anomalous diffraction de novo phasing using an X-ray-free electron laser
04 Nov 2016 | BioXFEL Publications | Contributor(s): Mark S. Hunter, Chun Hong Yoon, Hasan Demirci, Raymond G. Sierra, E. Han Dao, Radman Ahmadi, Fulya Aksit, Andrew L. Aquila, Halilibrahim Ciftci, Serge Guillet, Matt J. Hayes, Thomas J. Lane, Meng Liang, Ulf Lundström, Jason E. Koglin, Paul Mgbam, Yashas Rao, Lindsey Zhang, Soichi Wakatsuki, James Holton, Sébastien Boutet
http://dx.doi.org/10.1038/ncomms13388 Mark S. Hunter and Chun Hong Yoon and Hasan DeMirci and Raymond G. Sierra and E. Han Dao and Radman Ahmadi and Fulya Aksit and Andrew L. Aquila and Halilibrahim Ciftci and Serge Guillet and Matt J. Hayes and Thomas J. Lane and Meng Liang and Ulf Lundström and …
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Advanced Transmission Electron Microscopy
25 Oct 2016 | BioXFEL Publications | Contributor(s): Jian Min Zuo, John Spence
http://dx.doi.org/10.1007/978-1-4939-6607-3 book Zuo, J.M. & Spence, J.C.H., 2017. Advanced Transmission Electron Microscopy. Available at: http://dx.doi.org/10.1007/978-1-4939-6607-3.
Jian Min Zuo and John C.H. Spence
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XFEL Game - Windows Download
25 Oct 2016 | Downloads | Contributor(s): fiacre
Windows Version
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The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
19 Oct 2016 | BioXFEL Publications | Contributor(s): Petra Edlund, Heikki Takala, Elin Claesson, Léocadie Henry, Robert Dods, Heli Lehtivuori, Matthijs Panman, Kanupriya Pande, Thomas White, Takanori Nakane, Oskar Berntsson, Emil Gustavsson, Petra Båth, Vaibhav Modi, Shatabdi Roy-Chowdhury, James D Zook, Peter Berntsen, Suraj Pandey, Ishwor Poudyal, Jason Tenboer, Christopher Kupitz, Anton Barty, Petra Fromme, Jake D. Koralek, Tomoyuki Tanaka, John Spence, Mengning Liang, Mark S. Hunter, Sebastien Boutet, Eriko Nango, Keith Moffat, Gerrit Groenhof, Janne Ihalainen, Emina A. Stojković, Marius Schmidt, Sebastian Westenhoff
http://dx.doi.org/10.1038/srep35279 Petra Edlund and Heikki Takala and Elin Claesson and Léocadie Henry and Robert Dods and Heli Lehtivuori and Matthijs Panman and Kanupriya Pande and Thomas White and Takanori Nakane and Oskar Berntsson and Emil Gustavsson and Petra Båth and Vaibhav Modi and …
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Flexibility and design: conformational heterogeneity along the evolutionary trajectory of a redesigned ubiquitin
19 Oct 2016 | BioXFEL Publications | Contributor(s): Justin T Biel, Michael Thompson, Christian N Cunningham, Jacob E Corn, James Fraser
http://dx.doi.org/10.1101/081646 Although protein design has been used to introduce new functions, designed variants generally only function as well as natural proteins after rounds of laboratory evolution. One possibility for this pattern is that designed mutants frequently sample nonfunctional …
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Learned motivation drives circadian physiology in the absence of the master circadian clock
12 Oct 2016 | BioXFEL Publications | Contributor(s): Oliver Rawashdeh, Shannon J. Clough, Randall L. Hudson, Margarita L. Dubocovich
http://dx.doi.org/10.1096/fj.201600926r journal-article Rawashdeh, O. et al., 2017. Learned motivation drives circadian physiology in the absence of the master circadian clock. The FASEB Journal, 31(1), pp.388–399. Available at: http://dx.doi.org/10.1096/fj.201600926r.
Oliver Rawashdeh and …
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Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex
12 Oct 2016 | BioXFEL Publications | Contributor(s): Artem Y Lyubimov, Monarin Uervirojnangkoorn, Oliver B Zeldin, Qiangjun Zhou, Minglei Zhao, Aaron S Brewster, Tara Michels-Clark, James Holton, Nicholas K Sauter, William I Weis, Axel T Brunger
X-ray free electron lasers (XFELs) reduce the effects of radiation damage on macromolecular diffraction data and thereby extend the limiting resolution. Previously, we adapted classical post-refinement techniques to XFEL diffraction data to produce accurate diffraction data sets from a limited …
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Successful Strategies to Determine High-Resolution Structures of GPCRs
11 Oct 2016 | BioXFEL Publications | Contributor(s): Jin Xiang, Eugene Chun, Chang Liu, Liang Jing, Zina Al-Sahouri, Lan Zhu, Wei Liu
journal-article http://dx.doi.org/10.1016/j.tips.2016.09.009 NSF-STC Biology with X-ray Lasers (NSF-1231306) Xiang, J. et al., 2016. Successful Strategies to Determine High-Resolution Structures of GPCRs. Trends in Pharmacological Sciences, 37(12), pp.1055–1069. Available at: …
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Reconstruction of an object from diffraction intensities averaged over multiple object clusters
29 Sep 2016 | BioXFEL Publications | Contributor(s): J P J Chen, R D Arnal, A J Morgan, R J Bean, K R Beyerlein, H N Chapman, P J Bones, R P Millane, R A Kirian
http://dx.doi.org/10.1088/2040-8978/18/11/114003 J P J Chen and R D Arnal and A J Morgan and R J Bean and K R Beyerlein and H N Chapman and P J Bones and R P Millane and R A Kirian Chen, J.P.J. et al., 2016. Reconstruction of an object from diffraction intensities averaged over multiple object …
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De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure
27 Sep 2016 | BioXFEL Publications | Contributor(s): Jacques-Philippe Colletier, Michael R. Sawaya, Mari Gingery, Jose A. Rodriguez, Duilio Cascio, Aaron S. Brewster, Tara Michels-Clark, Robert H. Hice, Nicolas Coquelle, Sébastien Boutet, Garth J. Williams, Marc Messerschmidt, Daniel P. DePonte, Raymond G. Sierra, Hartawan Laksmono, Jason E. Koglin, Mark S. Hunter, Hyun-Woo Park, Monarin Uervirojnangkoorn, Dennis K. Bideshi, Axel T. Brunger, Brian A. Federici, Nicholas K. Sauter, David S. Eisenberg
http://dx.doi.org/10.1038/nature19825 Jacques-Philippe Colletier and Michael R. Sawaya and Mari Gingery and Jose A. Rodriguez and Duilio Cascio and Aaron S. Brewster and Tara Michels-Clark and Robert H. Hice and Nicolas Coquelle and Sébastien Boutet and Garth J. Williams and Marc Messerschmidt and …
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Angular correlations of photons from solution diffraction at a free-electron laser encode molecular structure
26 Sep 2016 | BioXFEL Publications | Contributor(s): Derek Mendez, Herschel Watkins, Shenglan Qiao, Kevin S. Raines, Thomas J. Lane, Gundolf Schenk, Garrett Charles Nelson, GANESH SUBRAMANIAN, Kensuke Tono, Yasumasa Joti, Makina Yabashi, Daniel Ratner, Sebastian Doniach
During X-ray exposure of a molecular solution, photons scattered from the same molecule are correlated. If molecular motion is insignificant during exposure, then differences in momentum transfer between correlated photons are direct measurements of the molecular structure. In conventional small- …
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Native phasing of x-ray free-electron laser data for a G protein-coupled receptor
23 Sep 2016 | BioXFEL Publications | Contributor(s): Alexander Batyuk, Lorenzo Galli, Andrii Ishchenko, Gye Won Han, Cornelius Gati, Petr A. Popov, Ming-Yue Lee, Benjamin Stauch, Thomas A. White, Anton Barty, Andrew Aquila, Mark S. Hunter, Mengning Liang, Sébastien Boutet, Mengchen Pu, Zhi-jie Liu, Garrett Charles Nelson, Daniel James, Chufeng Li, Yun Zhao, John Spence, Wei Liu, Petra Fromme, Vsevolod Katritch, Uwe Weierstall, Raymond C. Stevens, Vadim Cherezov
http://dx.doi.org/10.1126/sciadv.1600292 Alexander Batyuk and Lorenzo Galli and Andrii Ishchenko and Gye Won Han and Cornelius Gati and Petr A. Popov and Ming-Yue Lee and Benjamin Stauch and Thomas A. White and Anton Barty and Andrew Aquila and Mark S. Hunter and Mengning Liang and Sébastien …
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Spectral Hole Burning in Cyanobacterial Photosystem I with P700 in Oxidized and Neutral States
23 Sep 2016 | BioXFEL Publications | Contributor(s): Nicoleta Herascu, Mark S. Hunter, Golia Shafiei, Mehdi Najafi, T. Wade Johnson, Petra Fromme, Valter Zazubovich
http://dx.doi.org/10.1021/acs.jpcb.6b07803 Nicoleta Herascu and Mark S. Hunter and Golia Shafiei and Mehdi Najafi and T. Wade Johnson and Petra Fromme and Valter Zazubovich Herascu, N. et al., 2016. Spectral Hole Burning in Cyanobacterial Photosystem I with P700 in Oxidized and Neutral States. The …
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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
20 Sep 2016 | BioXFEL Publications | Contributor(s): Andrii Ishchenko, Vadim Cherezov, Wei Liu
http://dx.doi.org/10.3791/54463 Andrii Ishchenko and Vadim Cherezov and Wei Liu Ishchenko, A., Cherezov, V. & Liu, W., 2016. Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. Journal of Visualized Experiments, (115). Available at: …
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Crystallographic Methods of Structural Biology
18 Sep 2016 | Courses | Contributor(s): Robert H Blessing, Edward Snell
The goal of the course is that students acquire sufficient knowledge and understanding of the basic principles of biomolecular crystal structure analysis that they will be able to comfortably, and with interest and insight, read and comprehend the articles in Volume F of the International Tables …
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Establishing nonlinearity thresholds with ultraintense X-ray pulses
13 Sep 2016 | BioXFEL Publications | Contributor(s): Jakub Szlachetko, Joanna Hoszowska, Jean-Claude Dousse, Maarten Nachtegaal, Wojciech Błachucki, Yves Kayser, Jacinto Sà, Marc Messerschmidt, Sebastien Boutet, Garth J. Williams, Christian David, Grigory Smolentsev, Jeroen A. Van Bokhoven, Bruce D. Patterson, Thomas J. Penfold, Gregor Knopp, Marek Pajek, Rafael Abela, Christopher J. Milne
http://dx.doi.org/10.1038/srep33292 Jakub Szlachetko and Joanna Hoszowska and Jean-Claude Dousse and Maarten Nachtegaal and Wojciech Błachucki and Yves Kayser and Jacinto Sà and Marc Messerschmidt and Sebastien Boutet and Garth J. Williams and Christian David and Grigory Smolentsev and Jeroen A. …
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X-ray laser-induced electron dynamics observed by femtosecond diffraction from nanocrystals of Buckminsterfullerene
09 Sep 2016 | BioXFEL Publications | Contributor(s): Brian Abbey, Ruben A. Dilanian, Connie Darmanin, Rebecca A. Ryan, Corey T. Putkunz, Andrew V. Martin, David Wood, Victor Streltsov, Michael W. M. Jones, Naylyn Gaffney, Felix Hofmann, Garth J. Williams, Sébastien Boutet, Marc Messerschmidt, M. Marvin Seibert, Sophie Williams, Evan Curwood, Eugeniu Balaur, Andrew G. Peele, Keith A. Nugent, Harry M. Quiney
http://dx.doi.org/10.1126/sciadv.1601186 Brian Abbey and Ruben A. Dilanian and Connie Darmanin and Rebecca A. Ryan and Corey T. Putkunz and Andrew V. Martin and David Wood and Victor Streltsov and Michael W. M. Jones and Naylyn Gaffney and Felix Hofmann and Garth J. Williams and Sébastien Boutet …
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Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex
09 Sep 2016 | BioXFEL Publications | Contributor(s): Philip J. Robinson, Michael J. Trnka, David Bushnell, Ralph E. Davis, Pierre-Jean Mattei, Alma L. Burlingame, Roger Kornberg
http://dx.doi.org/10.1016/j.cell.2016.08.050 Philip J. Robinson and Michael J. Trnka and David A. Bushnell and Ralph E. Davis and Pierre-Jean Mattei and Alma L. Burlingame and Roger D. Kornberg Robinson, P.J. et al., 2016. Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex. …
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Ghost imaging with x rays
07 Sep 2016 | BioXFEL Publications | Contributor(s): Dilano Saldin
journal-article http://dx.doi.org/10.1103/physics.9.103 Saldin, D., 2016. Ghost imaging with x rays. Physics, 9. Available at: http://dx.doi.org/10.1103/physics.9.103.
Dilano Saldin
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Frontiers of free-electron laser science II
06 Sep 2016 | BioXFEL Publications | Contributor(s): Roger Falcone, Michael Dunne, Henry Chapman, Makina Yabashi, Kiyoshi Ueda
http://dx.doi.org/10.1088/0953-4075/49/18/180201 journal-article Falcone, R. et al., 2016. Frontiers of free-electron laser science II. Journal of Physics B: Atomic, Molecular and Optical Physics, 49(18), p.180201. Available at: http://dx.doi.org/10.1088/0953-4075/49/18/180201.
Roger Falcone and …
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Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers
29 Aug 2016 | BioXFEL Publications | Contributor(s): George Douglas Calvey, Andrea Katz, Chris B. Schaffer, Lois Pollack
http://dx.doi.org/10.1063/1.4961971 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Calvey, G.D. et al., 2016. Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers. Structural Dynamics, 3(5), p.054301. Available at: …
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The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction
29 Aug 2016 | BioXFEL Publications | Contributor(s): Ying Wu, Xiaohong Zhou, Christopher Barnes, Maria DeLucia, Aina E Cohen, Angela M Gronenborn, Jinwoo Ahn, Guillermo Calero
journal-article http://dx.doi.org/10.1038/nsmb.3284 NSF-STC Biology with X-ray Lasers (NSF-1231306) Wu, Y. et al., 2016. The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction. Nature Structural & Molecular Biology, 23(10), pp.933–940. Available …