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XrayView
24 Jun 2016 | Tools | Contributor(s): George Phillips
XRayView uses interactive computer graphics to introduce basic concepts of X-ray diffraction by crystals.
https://bioxfel.org/resources/1178
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Oct 28 2015
Arizona State University Biophysics Seminar - Tamir Gonen
MicroED - 3D electron crystallography Dr. Tamir Gonen We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in …
https://bioxfel.org/index.php?option=com_events&task=details&id=14
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Crystallography with an XFEL
08 May 2014 | Seminars | Contributor(s): Marc Messerschmidt
Dr. Marc Messerschmidt discusses the design and operation of the X-ray free electron laser at the LCLS. Be sure to grab the attached MP3 audio file to follow along. Topics discussed include: - XFEL …
https://bioxfel.org/resources/25
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Small Angle X-Ray Scattering as a Complementary Tool for High-Throughput Structural Studies
21 May 2014 | Publications | Contributor(s): Tom Grant, Edward Snell
Structural crystallography and nuclear magnetic resonance (NMR) spectroscopy are the predominant techniques for understanding the biological world on a molecular level. Crystallography is constrained …
https://bioxfel.org/resources/36
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De novo protein crystal structure determination from X-ray free-electron laser data
08 Jan 2014 | Publications | Contributor(s): R. L. Shoeman, L. Foucar, I. Schlichting, S. Boutet, R. B. Doak, K. Nass, S. Botha, G. J. Williams, J. E. Koglin, T. R. Barends, Mark Messerschmidt
The determination of protein crystal structures is hampered by the need for macroscopic crystals. X-ray free-electron lasers (FELs) provide extremely intense pulses of femtosecond duration, which …
https://bioxfel.org/resources/238
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Protein structural ensembles are revealed by redefining X-ray electron density noise
06 Jan 2014 | Publications | Contributor(s): T. Alber, J. S. Fraser, P. T. Lang, James Holton
To increase the power of X-ray crystallography to determine not only the structures but also the motions of biomolecules, we developed methods to address two classic crystallographic problems: …
https://bioxfel.org/resources/282
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Serial femtosecond crystallography of G protein-coupled receptors
19 Dec 2013 | Publications | Contributor(s): K. N. Rendek, G. J. Williams, W. Liu, R. C. Stevens, N. A. Zatsepin, J. E. Koglin, M. Caffrey, S. Boutet, D. Wang, R. A. Kirian, C. Wang, I. Grotjohann, T. A. White, K. R. Beyerlein, G. W. Han, A. Barty, S. Basu, D. Wacker, V. Katritch, C. Gati, M. M. Seibert, S. T. Shah, Henry Chapman, Vadim Cherezov, Petra Fromme, James Holton, Christopher Kupitz, Applications Manager, Mark Messerschmidt, Garrett Charles Nelson, John Spence, Uwe Weierstall
X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and …
https://bioxfel.org/resources/290
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The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA
23 Jul 2013 | Publications | Contributor(s): S. Corretore, H. Tsuruta, E. M. Phizicky, E. J. Grayhack, J. R. Wolfley, E. Quartley, Tom Grant, Joseph Luft, Edward Snell
Eukaryotic glutaminyl-tRNA synthetase (GlnRS) contains an appended N-terminal domain (NTD) whose precise function is unknown. Although GlnRS structures from two prokaryotic species are known, no …
https://bioxfel.org/resources/301
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Crystallographic data processing for free-electron laser sources
30 Jun 2013 | Publications | Contributor(s): N. A. Zatsepin, F. Stellato, T. A. White, A. Barty, R. A. Kirian, Henry Chapman, James Holton
A processing pipeline for diffraction data acquired using the `serial crystallography' methodology with a free-electron laser source is described with reference to the crystallographic analysis suite …
https://bioxfel.org/resources/236
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Three-dimensional single-particle imaging using angular correlations from X-ray laser data
30 Jun 2013 | Publications | Contributor(s): P. H. Zwart, B. K. Poon, H. G. Liu, Dilano Saldin, John Spence
Femtosecond X-ray pulses from X-ray free-electron laser sources make it feasible to conduct room-temperature solution scattering experiments far below molecular rotational diffusion timescales. …
https://bioxfel.org/resources/304
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Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature
25 Apr 2013 | Publications | Contributor(s): J. Hellmich, D. Milathianaki, U. Bergmann, R. J. Gildea, B. Lassalle-Kaiser, M. J. Bogan, H. Laksmono, P. H. Zwart, S. Gul, A. Miahnahri, J. Messinger, D. W. Schafer, N. K. Sauter, W. E. White, R. W. Grosse-Kunstleve, J. Hattne, D. Difiore, M. M. Seibert, T. C. Weng, R. G. Sierra, A. Zouni, J. Yano, G. J. Williams, P. Glatzel, S. Boutet, J. Kern, D. Sokaras, A. Lampe, V. K. Yach, R. Tran, N. Echols, J. E. Koglin, C. Glockner, M. J. Latimer, S. Koroidov, R. Alonso-Mori, A. R. Fry, J. Sellberg, G. Han, P. D. Adams, Mark Messerschmidt, Ranga Setlur
Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of …
https://bioxfel.org/resources/293
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Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser
10 Jan 2013 | Publications | Contributor(s): I. Grotjohann, D. P. DePonte, S. Bajt, N. A. Zatsepin, L. Redecke, C. Caleman, T. R. Barends, L. C. Johansson, G. Katona, T. Barth, R. Koopmann, A. Barty, J. Steinbrener, D. Wang, M. J. Bogan, S. Kassemeyer, D. Arnlund, L. Lomb, R. Neutze, C. Betzel, K. Nass, G. J. Williams, M. M. Seibert, T. A. White, R. B. Doak, I. Schlichting, D. Rehders, A. Aquila, S. Boutet, N. Timneanu, M. Duszenko, H. Fleckenstein, L. Galli, R. L. Shoeman, T. C. Chao, A. V. Martin, F. Stellato, M. Liang, S. Mogk, R. A. Kirian, Henry Chapman, Matthias Frank, Petra Fromme, Mark S. Hunter, Christopher Kupitz, Mark Messerschmidt, John Spence, Uwe Weierstall
https://bioxfel.org/resources/273
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Crystallography: Sources of inspiration
31 Dec 2012 | Publications | Contributor(s): S. McSweeney, Petra Fromme
2014.
https://bioxfel.org/resources/237
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High-resolution protein structure determination by serial femtosecond crystallography
19 Jul 2012 | Publications | Contributor(s): H. T. Philipp, D. Arnlund, T. A. White, P. Hart, M. M. Seibert, K. Nass, C. Kenney, S. Boutet, J. Pines, G. Haller, M. Hromalik, R. L. Shoeman, N. Timneanu, A. Barty, I. Schlichting, D. Schafer, L. J. Koerner, R. A. Kirian, G. Katona, S. M. Gruner, P. A. Montanez, M. Liang, C. Caleman, J. Morse, F. Stellato, L. Redecke, R. Herbst, N. A. Zatsepin, D. P. DePonte, T. R. Barends, A. Aquila, M. W. Tate, L. C. Johansson, G. J. Williams, C. Y. Hampton, S. Kassemeyer, J. Steinbrener, N. van Bakel, M. J. Bogan, R. Neutze, A. V. Martin, J. Defever, D. Wang, W. Ghonsalves, L. Lomb, R. B. Doak, Henry Chapman, Petra Fromme, Mark S. Hunter, Christopher Kupitz, Mark Messerschmidt, John Spence, Uwe Weierstall
Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding …
https://bioxfel.org/resources/254
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In vivo protein crystallization opens new routes in structural biology
29 Feb 2012 | Publications | Contributor(s): T. Ekeberg, D. Rolles, R. Koopmann, C. B. Wunderer, N. Kimmel, L. Redecke, J. Schulz, H. Hirsemann, R. B. Doak, A. V. Martin, G. Weidenspointner, S. Stern, C. Y. Hampton, J. D. Bozek, M. Liang, L. Foucar, A. Barty, F. Stellato, S. Bajt, C. Bostedt, P. Holl, M. Barthelmess, D. Rehders, R. Hartmann, B. Rudek, N. Coppola, H. Graafsma, T. A. White, L. Lomb, J. Hajdu, R. A. Kirian, A. Rudenko, G. J. Williams, S. Kassemeyer, C. Reich, J. Andreasson, M. J. Bogan, K. Nass, T. Stehle, F. R. Maia, R. L. Shoeman, C. Betzel, N. Timneanu, A. Aquila, L. Struder, J. Ullrich, H. Soltau, D. P. Deponte, R. G. Sierra, B. Erk, H. Fleckenstein, S. W. Epp, L. Gumprecht, J. Davidsson, M. M. Seibert, A. Hartmann, X. Wang, G. Hauser, S. Boutet, C. Caleman, I. Schlichting, M. Duszenko, K. Cupelli, Henry Chapman, Petra Fromme, Mark S. Hunter, Mark Messerschmidt, John Spence, Uwe Weierstall
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis.
https://bioxfel.org/resources/258
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Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals
28 Feb 2011 | Publications | Contributor(s): L. Lomb, T. A. White, A. Barty, F. R. Maia, R. A. Kirian, A. Aquila, X. Wang, A. V. Martin, Henry Chapman, Petra Fromme, James Holton, Mark S. Hunter, Marius Schmidt, John Spence
A complete set of structure factors has been extracted from hundreds of thousands of femtosecond single-shot X-ray microdiffraction patterns taken from randomly oriented nanocrystals. The method of …
https://bioxfel.org/resources/302
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Femtosecond X-ray protein nanocrystallography
02 Feb 2011 | Publications | Contributor(s): D. Pietschner, B. Nilsson, M. Adolph, J. Schulz, T. A. White, S. Bajt, G. J. Williams, M. M. Seibert, A. Rudenko, L. Gumprecht, F. R. Maia, N. Timneanu, C. Reich, S. Stern, H. Graafsma, R. Hartmann, K. U. Kuhnel, J. D. Bozek, A. Barty, R. Andritschke, D. Rupp, C. Bostedt, M. Bott, G. Schaller, T. R. Barends, I. Grotjohann, S. Herrmann, D. P. DePonte, B. Erk, L. Struder, G. Hauser, P. Holl, J. Krzywinski, H. Soltau, R. Neutze, M. Liang, A. Rocker, F. Krasniqi, N. Kimmel, C. D. Schroter, S. Schorb, H. Gorke, R. G. Sierra, K. Nass, X. Wang, J. Andreasson, R. A. Kirian, D. Rolles, B. Rudek, M. Barthelmess, A. Aquila, J. Ullrich, T. Gorkhover, I. Andersson, S. Boutet, G. Potdevin, C. Y. Hampton, M. Svenda, A. V. Martin, L. Lomb, F. Schopper, O. Jonsson, J. Hajdu, S. W. Epp, H. Hirsemann, R. L. Shoeman, L. Foucar, A. Homke, R. B. Doak, D. Starodub, N. Coppola, C. Caleman, M. J. Bogan, S. Marchesini, G. Weidenspointner, I. Schlichting, Henry Chapman, Matthias Frank, Petra Fromme, Stefan Hau-Riege, James Holton, Mark S. Hunter, Mark Messerschmidt, Marius Schmidt, John Spence, Uwe Weierstall
X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the …
https://bioxfel.org/resources/252
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Reply to 'Contradictions in X-ray structures of intermediates in the photocycle of photoactive yellow protein'
31 Dec 2010 | Publications | Contributor(s): H. Ihee, Y. O. Jung, K. Moffat, V. Srajer, J. Kim, Daniel Tsung-tai Lee, Marius Schmidt
2014.
https://bioxfel.org/resources/287